Journal: PLoS ONE

Article Title: Melanocyte progenitor cells reside in human subcutaneous adipose tissue

doi: 10.1371/journal.pone.0256622

Figure Lengend Snippet: The expression levels of HMB45, MITF, MelEM, Mel2, MelanA, MATP, and tyrosinase (TYR) in undifferentiated and differentiated ADSCs, as well as NHEMs, were examined by immunofluorescence microscopy. All melanocytic markers except for TYR were detected.

Article Snippet: In addition, undifferentiated and differentiated ADSCs were fixed for 30 min in 4% paraformaldehyde at room temperature and blocked with PBS containing 0.1% Triton (Wako) and 10% goat serum (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for 1 h at room temperature followed by a 1 h incubation at 37°C with primary antibodies against microphthalmia-associated transcription factor (MITF, 1:250, Abcam Biotechnology), Melan A (1:1000, Abcam Biotechnology), Mel2 (1:500, Abcam Biotechnology), MelEM (1:1; Developmental Studies Hybridoma Bank), MATP (1:400; Abcam Biotechnology), and Tyrosinase (TYR, 1:250, Abcam Biotechnology).

Techniques: Expressing, Immunofluorescence, Microscopy

Journal: Scientific Reports

Article Title: TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells

doi: 10.1038/srep23775

Figure Lengend Snippet: All pairs of F/R primers used for real-time qPCR.

Article Snippet: Rabbit polyclonal antibodies against MMP9, SNAI1, p16 and Vimentin were bought from BioSynthesis (Beijing, China), whilst other rabbit antibodies against α-Catenin, CDK2, CDK6, Cyclin D1, SNAI2, FN1, Rb1, p53 and p21 were purchased from BosTer (Wuhan, China).

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: IL-7R blockade reduces post-myocardial infarction-induced atherosclerotic plaque inflammation in ApoE −/- mice

doi: 10.1016/j.bbrep.2019.100647

Figure Lengend Snippet: Monoclonal antibody blocking of IL-7R post-MI. Aortic sinus plaque lipid (A), macrophage (D), or T cells stained area (G) in mice subjected to MI and injected with IgG control (B and E) or IL-7R mAb (C and F). Scale bar = 0.1 mm (A) * p = 0.004; (B) * p = 0.014 t -test; N = 4 each. Splenocytes were analyzed using fluorescent staining and flow cytometry for percentage of macrophage (H) or T cells (I). Gating for CD11b + F4/80 + macrophages as shown in . Gating for T cells as shown in .

Article Snippet: IL-7 protein expression was assessed using an IL-7 monoclonal antibody (Boster Bio) in 2 slides from each heart at 0.1 mm intervals with 2 sections of 0.01 mm thickness per slide.

Techniques: Blocking Assay, Staining, Injection, Control, Flow Cytometry